The results of the direct RAST (Radio-Allergo-Sorbent-Test) procedure used to standardise own-prepared pollen extract of the Dactylis glomerata grass are presented. Based on the performed standardisation, a system was set up for binding allergens to the stable inert carriers. The results of examination of the quality of D. glomerata allergen, bound covalently to paper disc, are presented also. Evaluation was made in INEP laboratories by comparing the D. glomerata allergen-bound discs and the corresponding discs of Phadebas, Pharmacia Uppsala, confirming a high degree of correlation in the direct RAST procedure.