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Nucleic Acids Res. 1992 Dec 25;20(24):6565-73.

Solid-phase synthesis of branched oligoribonucleotides related to messenger RNA splicing intermediates.

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  • 1Department of Chemistry, Erindale College, University of Toronto, Ontario, Canada.

Abstract

The chemical synthesis of oligoribonucleotides containing vicinal (2'-5')- and (3'-5')-phosphodiester linkages is described. The solid-phase method, based on silyl-phosphoramidite chemistry, was applied to the synthesis of a series of branched RNA [(Xp)nA2' (pN)n3'(pN)n] related to the splicing intermediates derived from Saccharomyces cerevisiae rp51a pre-messenger RNA. The branched oligonucleotides have been thoroughly characterized by nucleoside and branched nucleotide composition analysis. Branched oligoribonucleotides will be useful in the study of messenger RNA splicing and in determining the biological role of RNA 'lariats' and 'forks' in vivo.

PMID:
1480476
[PubMed - indexed for MEDLINE]
PMCID:
PMC334572
Free PMC Article
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