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1: PCR Methods Appl. 1992 Nov;2(2):144-8.Click here to read Links

A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

Borson ND, Salo WL, Drewes LR.

Department of Biochemistry and Molecular Biology, School of Medicine, University of Minnesota, Duluth 55812.

We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.

PMID: 1477669 [PubMed - indexed for MEDLINE]