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Somat Cell Mol Genet. 1992 Sep;18(5):443-50.

Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor.

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  • 1Molecular Hematology Branch, National Heart Lung and Blood Institute, Bethesda, Maryland 20892.


The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length and the deduced amino acid sequence bears 78.2% homology with the reported human sequence. This murine cDNA was subcloned into a retroviral-based expression vector, LmLSN1, and transfected into 3T3 cells. The production of functional LDL receptor was confirmed by ligand binding of DiI-LDL cholesterol.

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