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Trends Genet. 2004 Feb;20(2):59-62.

To knockout in 129 or in C57BL/6: that is the question.

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  • 1Mental Health Research Institute and Neuroscience Program, University of Michigan, Ann Arbor, MI 48109, USA.


Traditionally, knockout experiments are performed in ES cells derived from the 129 mouse strain, followed by backcrossing with the more robust C57BL/6 strain. C57BL/6-derived ES cells have only occasionally been used in this process. We compared C57BL/6- with 129-derived ES cells directly and reviewed the literature. We found that, although some steps are less efficient, the advantages of C57BL/6 mice more than compensate for these drawbacks.

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