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Bioinformatics. 2004 Jan 22;20(2):271-3.

ROSO: optimizing oligonucleotide probes for microarrays.

Author information

  • 1Biologie Fonctionnelle, Insectes et Interactions (BF2I), UMR INRA/INSA de Lyon, 69621 Villeurbanne, France. nancie.reymond@jouy.inra.fr

Abstract

ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set.

AVAILABILITY:

The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors.

SUPPLEMENTARY INFORMATION:

http://pbil.univ-lyon1.fr/roso

PMID:
14734320
[PubMed - indexed for MEDLINE]
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