UMR INRA UHP 1136, Interaction Arbres Microorganismes, Université Henri Poincaré, Faculté des Sciences, BP 239, 54506 Vandoeuvre Cédex, France. j2p@scbiol.uhp-nancy.fr
A single rabbit has been immunized with a mixture of six different proteins: green fluorescent protein, ferredoxin:NADP oxidoreductase, NADPH:thioredoxin reductase, thioredoxin f, fructose bisphosphatase and cyclophilin. Individual IgGs were re-purified from the serum through specific interaction on small columns generated by grafting the antigen on a CNBr-Sepharose matrix. The specificity of the isolated IgG was checked by Western-blot analysis. Purified and specific IgG fractions were obtained with all antigens except thioredoxin f.