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1: Biochem Biophys Res Commun. 1992 Dec 15;189(2):697-704.Click here to read Links

Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1.

Ishizaka Y, Kangawa K, Minamino N, Ishii K, Takano S, Eto T, Matsuo H.

Department of Biochemistry, Miyazaki Medical College, Japan.

In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-type natriuretic peptide (CNP) precursor. CNP mRNA was also detected in the THP-1 cells, and expression of CNP gene and CNP concentration in the culture medium was found to be highly augmented by the stimulation of phorbol ester. Production of CNP in THP-1 cells suggests that CNP also functions as a local regulator in the blood cell-vascular system, although CNP has previously been recognized as a neuropeptide functioning in the central nervous system.

PMID: 1472040 [PubMed - indexed for MEDLINE]