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J Clin Microbiol. 2004 Jan;42(1):421-5.

Strengths and limitations of commercial tests for hepatitis C virus RNA quantification.

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  • 1Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA.

Abstract

The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.

PMID:
14715796
PMCID:
PMC321650
[PubMed - indexed for MEDLINE]
Free PMC Article
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