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Appl Environ Microbiol. 2004 Jan;70(1):610-2.

Simple and reliable method to precipitate proteins from bacterial culture supernatant.

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  • 1CREATOGEN AG, D-86156 Augsburg, Germany.

Abstract

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

PMID:
14711696
[PubMed - indexed for MEDLINE]
PMCID:
PMC321231
Free PMC Article

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