Immunohistochemical localization of p40 in psoriatic skin. The four panels are from frozen sections of human skin from psoriasis patients. A and B are IgG isotype controls in uninvolved and lesional skin, respectively. C and D are stained with mouse anti–human IL-12p40 as indicated. The arrow indicates morphology consistent with DCs. The color represents 3-amino-9-ethylcarbazole deposition at the site of antibody binding. Melanin appears in A as dark stain in the basal cell layer.

Expression of p19, p40, and p35 mRNA in monocyte-derived DCs. Monocytes were placed in culture at −144 h, differentiated to immature DCs, and then stimulated beginning at 0 h with TNF-α, IL-1β, IL-6, and PGE2 for the stated amount of time. The data bars (open and solid) represent relative gene expression of p19 (A), p40 (B), and p35 (C) from two separate experiments. Note differing abscissa scales.

Expression of p19, p40, and p35 mRNA in paired samples of uninvolved and lesional skin from psoriasis patients. ♦, indicates the arithmetic mean for each type of specimen and is not connected by a line. A line connects the paired samples taken from each patient's nonlesional and psoriatic skin. Data is presented as the ratio of PCR yield, in picograms, of p19 (A), p40 (B), and p35 (C) normalized to the yield of hARP. Note the logarithmic scale of the abscissa.

p19 and p40 gene expression in dispase split skin and dermally derived inflammatory cells. (A) Dermis from psoriasis lesions has greater p19 and p40 mRNA than epidermis. The data are from one representative experiment. A repeat run produced almost identical results. RNA was isolated from dispase split lesional skin taken from psoriatic plaques from two patients. RT-PCR for the p19 and p40 genes was performed on the epidermis and dermis and gene expression levels are shown relative to the hARP gene. In two other patients, the dermis was cultured for an additional 2 d at 37°C and the cells exiting the cultured dermis (B, C, and D) were collected for subsequent quantitative gene expression for p19 using immunomagnetic beads coated with antibodies to CD3, CD11c, or CD83. In each case, two cell populations were obtained: one adherent to the beads expressing CD83, CD11c, or CD68 (B), and one nonadherent (C and D) that was used to monitor the depletion by FACS^®. For the latter, flow-through cells were analyzed for the presence of HLA-DR and CD83. (B) Total RNA was isolated and gene expression for p19 was assayed using TaqMan chemistry as described in Materials and Methods. Data are from one representative experiment. A repeat run produced almost identical results. (C, left) Side and forward scatter plots and gating (R1 for lymphocytes and R2 for DCs) for subsequent panels. (C, middle and C, right) Isotype controls. Cells depleted by isotype control (D, top, panel 1), anti-CD11c (D, top, panel 2), anti-CD83 (D, top, panel 3), and anti-CD3 (D, top, panel 4). Note that percentages represent upper right quadrant cell count (HLA-DR⁺ CD83⁺) as percent of total. Each panel has a plot of forward scatter versus CD3⁺ to demonstrate additional specificity of the depletion.