Abstract
The early involvement of marginal zone (MZ) B lymphocytes in T-independent immune responses is well established. In this study we compared the abilities of MZ and follicular (FO) B cells to collaborate with T cells. After immunization with soluble hen egg lysozyme, both MZ and FO B cells captured Ag and migrated to T cell areas in the response to hen egg lysozyme. MZ B cells were far superior to FO B cells in inducing CD4+ T cell expansion both in vitro and in vivo. MZ, but not FO, B cells, after interaction with T cells, differentiated into plasma cells, and in addition they stimulated Ag-specific CD4+ T cells to produce high levels of Th1-like cytokines upon primary stimulation in vitro. These results indicate that MZ B cells rapidly and effectively capture soluble Ag and activate CD4+ T cells to become effector T cells. The enhanced capacity of MZ B cells to prime T cells in this study appeared to be intrinsic to MZ B cells, as both MZ and FO B cell populations express an identical Ag receptor.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigen Presentation
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Antigens, CD / physiology
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / metabolism
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B7-1 Antigen / physiology
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B7-2 Antigen
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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Cell Differentiation / immunology
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Cell Division / immunology
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Cell Movement / immunology
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Cells, Cultured
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Clone Cells
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Cytokines / biosynthesis
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Endocytosis / immunology
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Epitopes, T-Lymphocyte / metabolism
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Interphase / immunology*
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Lymphocyte Activation / immunology*
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Lymphocyte Cooperation / immunology
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Membrane Glycoproteins / physiology
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Plasma Cells / cytology
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Spleen / cytology*
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Spleen / immunology*
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Th1 Cells / immunology
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Th1 Cells / metabolism
Substances
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Antigens, CD
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B7-1 Antigen
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B7-2 Antigen
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Cd86 protein, mouse
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Cytokines
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Epitopes, T-Lymphocyte
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Membrane Glycoproteins