Photometric assay for measuring the intracellular concentration of branched-chain amino acids in bacteria

Dina Petranovic; Ivan Mijakovic

doi:10.1016/j.mimet.2003.10.002

Photometric assay for measuring the intracellular concentration of branched-chain amino acids in bacteria

J Microbiol Methods. 2004 Jan;56(1):133-6. doi: 10.1016/j.mimet.2003.10.002.

Authors

Dina Petranovic¹, Ivan Mijakovic

Affiliation

¹ Laboratoire de Génétique Microbienne, CRJ-INRA, F-78352 CEDEX, Jouy en Josas, France. dina@jouy.inra.fr

PMID: 14706758
DOI: 10.1016/j.mimet.2003.10.002

Abstract

The changes in intracellular pool of branched-chain amino acids (BCAA) regulate different physiological processes in bacteria. Up to date, the only available photometric test for measuring BCAA concentration was adapted for blood and plasma samples in diagnostic purposes. We have modified this method for use on bacterial cells, and tested its applicability on several model organisms: Lactococcus lactis, Bacillus subtilis and Escherichia coli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids, Branched-Chain / analysis*
Bacillus subtilis / chemistry*
Escherichia coli / chemistry*
Lactococcus lactis / chemistry*
Photometry / methods*

Substances

Amino Acids, Branched-Chain