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    J Biomed Biotechnol. 2003;2003(5):299-307.

    Optimization of Rolling-Circle Amplified Protein Microarrays for Multiplexed Protein Profiling.

    Shao W, Zhou Z, Laroche I, Lu H, Zong Q, Patel DD, Kingsmore S, Piccoli SP.

    Abstract

    Protein microarray-based approaches are increasingly being used in research and clinical applications to either profile the expression of proteins or screen molecular interactions. The development of high-throughput, sensitive, convenient, and cost-effective formats for detecting proteins is a necessity for the effective advancement of understanding disease processes. In this paper, we describe the generation of highly multiplexed, antibody-based, specific, and sensitive protein microarrays coupled with rolling-circle signal amplification (RCA) technology. A total of 150 cytokines were simultaneously detected in an RCA sandwich immunoassay format. Greater than half of these proteins have detection sensitivities in the pg/ml range. The validation of antibody microarray with human serum indicated that RCA-based protein microarrays are a powerful tool for high-throughput analysis of protein expression and molecular diagnostics.

    PMID:
    14688416
    [PubMed - as supplied by publisher]
    PMCID:
    PMC521503
    Free PMC Article

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