Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
EMBO J. 2004 Jan 14;23(1):127-37. Epub 2003 Dec 18.

Roles of SWI/SNF and HATs throughout the dynamic transcription of a yeast glucose-repressible gene.

Author information

  • 1Program in Molecular and Cellular Biology, Department of Microbiology and Immunology, SUNY Downstate Medical Center, Brooklyn, NY, USA.

Abstract

Eucaryotic gene expression requires chromatin-remodeling activities. We show by time-course studies that transcriptional induction of the yeast glucose-regulated SUC2 gene is rapid and shows a striking biphasic pattern, the first phase of which is partly mediated by the general stress transcription factors Msn2p/Msn4p. The SWI/SNF ATP-dependent chromatin-remodeling complex associates with the promoter in a similar biphasic manner and is essential for both phases of transcription. Two different histone acetyltransferases, Gcn5p and Esa1p, enhance the binding of SWI/SNF to the promoter during early transcription and are required for optimal SUC2 induction. Gcn5p is recruited to SUC2 simultaneously with SWI/SNF, whereas Esa1p associates constitutively with the promoter. This study reveals an unusual transcription pattern of a metabolic gene and suggests a novel strategy by which distinct chromatin remodelers cooperate for the dynamic activation of transcription.

PMID:
14685262
[PubMed - indexed for MEDLINE]
PMCID:
PMC1271673
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk