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Biochem Biophys Res Commun. 2004 Jan 9;313(2):308-13.

Selective production of rat mutant selenoprotein W with and without bound glutathione.

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  • 1Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA.

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and electrospray ionization mass spectrometry (ESI MS) analysis of a 6x His-tagged recombinant form of rat mutant selenoprotein W (RMSW) reveals that aerobic growth conditions primarily produce a form of RMSW without bound glutathione (10,305 Da) whereas anaerobic conditions produce a glutathione-bound (305 Da) form (10,610 Da). Purification of RMSW was achieved with a procedure employing acetone precipitation and DEAE-cellulose chromatography, in addition to Ni-NTA agarose chromatography. Additional steps, including polyvalent metal ion binding (PMIB) resin chromatography and CM-cellulose chromatography, were necessary after elution from the Ni-NTA agarose column, in order to maintain solubility of the purified protein.

PMID:
14684161
[PubMed - indexed for MEDLINE]
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