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Genes Dev. 2003 Dec 15;17(24):3011-6. Epub 2003 Dec 17.

Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.

Author information

  • 1Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.

Abstract

MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield approximately 65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, approximately 22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

PMID:
14681208
[PubMed - indexed for MEDLINE]
PMCID:
PMC305252
Free PMC Article

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