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Microsc Res Tech. 2004 Jan 1;63(1):18-22.

Practical limits of resolution in confocal and non-linear microscopy.

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  • 1Instituto Gulbenkian de Ciencia, Oeiras, Portugal.


Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2-photon and second harmonic) microscopes. A modest degree of super-resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence gives little resolution improvement over widefield. However, practical non-linear microscopes do approach their theoretical resolution and therefore show no resolution disadvantage relative to confocal microscopes in spite of the longer excitation wavelength.

Copyright 2003 Wiley-Liss, Inc.

[PubMed - indexed for MEDLINE]
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