Dendrogram of XbaI PFGE profiles of B. multivorans isolates, showing percentages of similarity of banding patterns. Isolates were labeled by a letter(s) representing the hospital, a number representing the patient, and, where multiple isolates from the same patient were received, small Roman numerals. The flagellin type of each isolate is also given (last column).

Flagellin PCR-RFLP analysis of B. multivorans isolates. (a) fliC amplicons produced by isolates. The four different size classes (indicated above the lane numbers) obtained are shown. Amplicons from representatives of each of the different RFLP types (except type 2e) are included. Isolates were X4 (lane 1), F1 (lane 2), N1 (lane 3), P1 (lane 4), Y1v (lane 5), C1 (lane 6), X1 (lane 7), B1 (lane 8), M1 (lane 9), A3ii (lane 10), G1 (lane 11), W1 (lane 12), O1 (lane 13), and K1 (lane 14). Molecular size markers (123-bp ladder) are shown in lanes M. (b) RFLP types identified by using MspI as the restriction enzyme. The RFLP type assigned to each pattern is given above the lane number. Isolates were X4 (lane 1), E1 (lane 2), N1 (lane 3), X3 (lane 4), A2 (lane 5), Y1v (lane 6), C1 (lane 7), B1 (lane 8), M1 (lane 9), A3ii (lane 10), G1 (lane 11), W1 (lane 12), O1 (lane 13), and K1 (lane 14). A type 2e amplicon was also found (data not shown). Molecular size markers (123-bp ladder) are shown in lanes M.