Effect of age on CAP thresholds for nonexposed mice. Data for +/+ +/v and +/sh1 +/v mice were merged and designated +/v, and data for +/sh1 +/+ and +/+ +/+ mice merged and designated +/+. A. Mean CAP thresholds for +/v and +/+ mice aged between 5 and 6 weeks. * signifies frequencies at which thresholds for +/v mice were significantly greater than those of +/+ mice (p < 0.05). Error bars represent SEM. CAP thresholds were significantly raised at 3 kHz (+22 dB, p = 0.0103), 6 kHz (+17 dB, p = 0.0284), 24 kHz (+26 dB, p = 0.001), and 30 kHz (+40 dB, p < 0.0001) in +/v compared with +/+ mice. B, C. Mean CAP thresholds for +/+ (B) and +/v (C) mice aged either 5–6 weeks, 11–12 weeks, or 23–24 weeks of age. * signifies frequencies at which thresholds were significantly greater than those of 5–6-week-old mice (p < 0.05). Error bars represent SEM. CAP thresholds for +/+ mice did not change with age. In contrast, CAP thresholds in 23–24-week-old +/v mice were significantly greater than those of 5–6-week-old +/v mice at 15 kHz (+30 dB, p = 0.0002), 18 kHz (+44 dB, p < 0.0001), 24 kHz (+34 dB, p < 0.0001), and 30 kHz (+16 dB, p = 0.0491) demonstrating that the high-frequency hearing loss worsens with increasing age. No significant increase in thresholds was observed at the low frequencies (3 kHz, p = 0.943 and 6 kHz, p = 0.3677) between 5–6- and 23–24-week-old +/v mice.

Effect of genetic background on CAP thresholds of 11–12-week-old nonexposed mice. Mean CAP thresholds for +/+ and +/v mice with no genetic contribution from the sh1 background. At all frequencies tested there were no significant differences in CAP thresholds between +/+ and +/v mice (p > 0.05). Error bars represent SEM.

Effect of noise exposure on hair cell stereocilia. A, B. Scanning electron micrographs of the surface of OHCs, in the outermost row (row 3), 58% of the total length of the cochlear duct from the base. This region responds best to 12 kHz according to the frequency place map described by Ehret (1975). A. Nonexposed +/sh1 +/v mouse, 11–12 weeks of age. Stereocilia are upright and arranged in a tight V-shaped bundle. B. +/sh1+/v mouse, 11–12 weeks of age, a week after being exposed to 8–16 kHz delivered at 103 dB SPL for 2 h. Gaps within the ranks of stereocilia are present (arrowhead) as are splayed stereocilia that lean outward from the bundle (arrow). Scale bar = 2 µm. C, D. Histograms showing the mean number of stereocilia (C) and splayed stereocilia (D) in the outermost (tallest) rank. Only OHCs positioned in the row nearest the IHCs and 58% of the total length of the cochlear duct from the base were analyzed. Counts were made for nonexposed (black bars) and noise-exposed (white bars) +/+ +/+, +/+ +/v, +/sh1 +/+, and +/sh1 +/v mice a week after exposure. * indicates a significant difference (p < 0.05) between exposed and nonexposed mice. At the genotype level, only +/+ +/v mice had a significant increase in splayed stereocilia after noise exposure. However, the effect of noise on stereocilia splaying was highly significant for the group as a whole (p < 0.0001 using a log–linear model). Neither the amount of stereocilia loss nor the increase in number of splayed stereocilia differed significantly between genotypes (p > 0.1). Error bars represent the SEM.

CAP thresholds for 11–12-week-old nonexposed mice. A. Mean CAP thresholds for +/+ +/+, +/sh1 +/+, +/+ +/v, and +/sh1 +/v mice plotted against frequency scaled according to the frequency place map described by Ehret (1975). * signifies frequencies at which thresholds were significantly different from those of +/+ +/+ mice (p < 0.05). Error bars represent SEM. CAP thresholds for +/+ +/v mice were significantly raised compared with +/+ +/+ mice at both low (3 kHz, +31 dB, p = 0.002; 6 kHz, +36 dB, p < 0.001; and 9 kHz, +29 dB, p = 0.004) and high frequencies (15 kHz, +19 dB, p = 0.047; 18 kHz, +30 dB, p = 0.001; 24 kHz, +53 dB, p < 0.001; and 30 kHz, +66 dB, p < 0.001). Similarly, for +/sh1 +/v mice, thresholds were significantly raised at low (3 kHz, +29 dB, p = 0.002, 6 kHz, +27 dB, p = 0.003; and 9 kHz, +24 dB, p =0.007) and high frequencies (18 kHz, +17 dB, p = 0.041; 24 kHz, +40 dB, p < 0.001; and 30 kHz, +51 dB, p < 0.001) compared with +/+ +/+ mice. B–E. CAP thresholds for individual +/+ +/+ (B), +/sh1 +/+ (C), +/+ +/v (D), and +/sh1 +/v (E) mice. For reference, the mean CAP threshold for +/+ +/+ mice has been plotted in grey. Mice heterozygous for Cdh23^v show greater interanimal variability than mice wild type at this locus.

Hair cell counts for nonexposed mice. Histograms showing the mean number of IHCs and OHCs in rows 1, 2, and 3 per 100 µm. OHC row 1 is defined as the row nearest the IHCs and 3 the furthest. Counts were made for +/+ +/+ (A), +/sh1 +/+ (B), +/+ +/v (C), and +/sh1 +/v (D) mice at 14%, 33%, 58%, and 77% of the total length of the cochlear duct from the base. The frequencies that each of these locations responds best to are shown in brackets. * signifies a significant reduction in cell number compared with the number of cells in +/+ +/+ mice (*p < 0.05, **p < 0.001). Error bars represent SEM. ihc: inner hair cell; ohc1: outer hair cell in row 1; ohc2: outer hair cell in row 2; ohc3: outer hair cell in row 3.

Effect of noise exposure on CAP thresholds and hair cell numbers. A. Mean threshold shift a week after exposure to 8–16 kHz delivered at 103 dB SPL for 2 h for 11–12-week-old +/+ +/+, +/sh1 +/+, +/+ +/v, and +/sh1+/v mice. Threshold shifts were calculated by subtracting the mean threshold for exposed mice from that of nonexposed mice with the same genotype. They are plotted against frequency scaled according to the frequency place map described by Ehret (1975). Frequencies at which threshold shifts differ significantly (p < 0.05) from those of +/+ +/+ mice are signified by *. The shaded box marks the frequency of noise used to expose these mice. Error bars represent SEM. B–E. Histograms showing the mean loss of IHCs and OHCs a week after noise exposure for 11–12-week-old +/+ +/+ (B),+/sh1 +/+ (C), +/+ +/v (D), and +/sh1 +/v (E) mice. Hair cell loss was measured at 14%, 33%, 58%, and 77% of the total length of the cochlear duct from the base. The frequencies that each of these locations responds best to are shown in brackets. Hair cell loss was calculated by subtracting the mean number of cells in exposed mice from the mean number of cells in nonexposed control mice. * denotes a significant (p ≤ 0.01) increase in hair cell loss. Note that there was no significant loss of hair cells at the 58% (12 kHz) region of either +/+ +/v or +/sh1 +/v mice despite a large threshold shift (approximately 35 dB) at this frequency. Error bars represent SEM. ihc: inner hair cell; ohc1: outer hair cell in row 1, nearest the IHCs; ohc2: outer hair cell in row 2; ohc3: outer hair cell in row 3, furthest from the IHCs.

Effect of genetic background on CAP threshold shifts. Graph shows the mean shift in CAP threshold a week after exposure to 8–16 kHz delivered at 103 dB SPL for 2 h for 11–12-week-old +/+ and +/v mice with no genetic contribution from the sh1 background. Threshold shifts were calculated by subtracting the mean threshold for exposed mice from those of nonexposed mice. For +/+ mice, threshold shifts were significant at 12 kHz (+18 dB, p = 0.008), 15 kHz (+19 dB, p = 0.006), and 30 kHz (+17 dB, p = 0.016) but not at 3, 6, 9, 18, or 24 kHz. Similarly, for +/v mice threshold shifts were significant at 9 kHz (+13 dB, p = 0.012), 12 kHz (+16 dB, p = 0.002), 15 kHz (+19 dB, p < 0.001), 18 kHz (+17 dB, p = 0.002), 24 kHz (+21 dB, p < 0.001), and 30 kHz (+28 dB, p < 0.001) but not at 3 or 6 kHz compared with nonexposed mice. Threshold shifts did not significantly differ between +/+ and +/Cdh23^v-mice at any of the frequencies tested (3, 9, 12, 15, 18, 24, and 30 kHz; p > 0.1). The shaded box marks the frequency of noise exposed to these mice. Error bars represent SEM.