cis-hinokiresinol, a norlignan from Anemarrhena asphodeloides, inhibits angiogenic response in vitro and in vivo

Biol Pharm Bull. 2003 Dec;26(12):1721-4. doi: 10.1248/bpb.26.1721.

Abstract

cis-Hinokiresinol (CHR) is a norlignan constituent from Anemarrhena asphodeloides BUNGE (Liliaceae), which shows hyaluronidase inhibitory activity. In the present studies, we have demonstrated that CHR selectively inhibited endothelial cell proliferation compared with cancer cells, and especially basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF)-induced endothelial cell proliferation. Furthermore, endothelial cell migration and tube formation, two important steps in the angiogenic process, were also inhibited by CHR. Moreover, CHR reduced the vessel growth induced by VEGF in the mouse corneal neovascularization model. These results suggest that CHR may prove useful for the development of a novel angiogenesis inhibitor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemarrhena / chemistry*
  • Angiogenesis Inhibitors / adverse effects
  • Angiogenesis Inhibitors / isolation & purification
  • Angiogenesis Inhibitors / pharmacokinetics
  • Animals
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cell Line
  • Cell Movement / drug effects
  • Cornea / blood supply
  • Cornea / drug effects
  • Dose-Response Relationship, Drug
  • Endothelial Cells / drug effects
  • Endothelial Cells / physiology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism
  • Fibroblast Growth Factor 2 / antagonists & inhibitors
  • Fibroblast Growth Factor 2 / pharmacology
  • Hyaluronoglucosaminidase / antagonists & inhibitors
  • Hyaluronoglucosaminidase / pharmacokinetics
  • Lignans / adverse effects
  • Lignans / chemistry*
  • Lignans / isolation & purification
  • Lignans / pharmacokinetics*
  • Mice
  • Mice, Inbred BALB C
  • Neovascularization, Pathologic / drug therapy
  • Neovascularization, Pathologic / etiology
  • Neovascularization, Pathologic / prevention & control*
  • Pharmacognosy / methods
  • Phenols / adverse effects
  • Phenols / isolation & purification
  • Phenols / pharmacokinetics*
  • Rhizome / chemistry
  • Stereoisomerism
  • Tumor Cells, Cultured
  • Umbilical Veins / cytology
  • Umbilical Veins / drug effects

Substances

  • Angiogenesis Inhibitors
  • Lignans
  • Phenols
  • Fibroblast Growth Factor 2
  • hinokiresinol
  • Hyaluronoglucosaminidase