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Mol Cell Biol. 2003 Dec;23(24):9283-92.

Novel methyltransferase for modified uridine residues at the wobble position of tRNA.

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  • 1Department of Chemistry and Biochemistry, UCLA Molecular Biology Institute, University of California-Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.


We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.

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