Abstract
Regulation of membrane trafficking requires the concerted actions of rab proteins, their effectors and several phosphatidylinositol 3'-kinases. Rab7 is required for late endosomal transport and here we establish that the phosphatidylinositol 3'-kinase hVPS34 and its adaptor protein p150 are rab7 interacting partners. The hVPS34/p150 complex colocalized with rab7 on late endosomes and hVPS34 activity was dependent on nucleotide cycling of rab7. In addition, total cellular phosphatidylinositol 3'-phosphate levels were modulated by rab7 expression, suggesting that rab7 activation impacted kinase cycling to early endosomes. The data identify rab7 as an important regulator of late endosomal hVPS34 function and link rab7 to the regulation of phosphatidylinositol 3'-kinase cycling between early and late endosomes.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Biological Transport
-
Cell Line
-
Cricetinae
-
Endocytosis / physiology
-
Endosomes / metabolism
-
Endosomes / ultrastructure
-
Humans
-
Macromolecular Substances
-
Membrane Glycoproteins / metabolism
-
Phosphatidylinositol 3-Kinases / genetics
-
Phosphatidylinositol 3-Kinases / metabolism*
-
Protein Kinases / genetics
-
Protein Kinases / metabolism*
-
Viral Envelope Proteins / metabolism
-
rab GTP-Binding Proteins / genetics
-
rab GTP-Binding Proteins / metabolism*
-
rab7 GTP-Binding Proteins
Substances
-
G protein, vesicular stomatitis virus
-
Macromolecular Substances
-
Membrane Glycoproteins
-
Viral Envelope Proteins
-
rab7 GTP-Binding Proteins
-
rab7 GTP-binding proteins, human
-
Protein Kinases
-
p150 protein kinase
-
rab GTP-Binding Proteins