Electrophoresis. 2003 Nov;24(21):3563-76.

Microfluidic devices fabricated in poly(dimethylsiloxane) for biological studies.

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Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Abstract

This review describes microfluidic systems in poly(dimethylsiloxane) (PDMS) for biological studies. Properties of PDMS that make it a suitable platform for miniaturized biological studies, techniques for fabricating PDMS microstructures, and methods for controlling fluid flow in microchannels are discussed. Biological procedures that have been miniaturized into PDMS-based microdevices include immunoassays, separation of proteins and DNA, sorting and manipulation of cells, studies of cells in microchannels exposed to laminar flows of fluids, and large-scale, combinatorial screening. The review emphasizes the advantages of miniaturization for biological analysis, such as efficiency of the device and special insights into cell biology.

PMID:: 14613181; [PubMed - indexed for MEDLINE]

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GM 65364/GM/NIGMS NIH HHS/United States

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