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Science. 2003 Dec 5;302(5651):1727-36. Epub 2003 Nov 6.

A protein interaction map of Drosophila melanogaster.

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  • 1CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA.

Abstract

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

PMID:
14605208
[PubMed - indexed for MEDLINE]
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