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Appl Environ Microbiol. 2003 Nov;69(11):6949-53.

Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii.

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  • 1Department of Microbiology, Cornell University, Ithaca, New York 14853, USA. jun_zhu@njau.edu.cn

Abstract

An ultrasensitive bioassay system for the detection of N-acylhomoserine lactones (AHLs) was constructed in Agrobacterium tumefaciens by using the T7 expression system to overproduce the AHL receptor TraR. This strain detected many diverse AHLs, some at extremely low concentrations. We used this strain to detect for the first time AHLs made by Mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume Astragalus sinicus, a source of green manure throughout eastern Asia.

PMID:
14602662
[PubMed - indexed for MEDLINE]
PMCID:
PMC262303
Free PMC Article
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