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J Biochem Biophys Methods. 2004 Jan 30;58(1):15-24.

A high performance micro-dual-wavelength-spectrophotometer (MDWS).

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  • 1Fachbereich Biologie/Botanik der Universit├Ąt Marburg, D-35032 Marburg, Lahnberge, Germany.


The dual wavelength spectrophotometer (DWS) has proven to be the most sensitive device to monitor minute optical absorbance changes, which are inaccessible to conventional single or double beam spectrophotometers. The typical set ups, e.g. extensively used for Ca2+ or phytochrome measurements, are huge, expensive and cumbrous. Therefore, a novel high performance micro-dual-wavelength spectrophotometer (MDWS) was developed. It is miniaturized and no moving parts such as vibrational mirrors or rotating filter wheels involved. Its specifications are superior compared to the conventional set up being capable of detecting minute optical changes (reflection, absorbance, transmittance) at particular wavelengths.

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