Chromosomal distribution of genes expressed significantly differently in ES cell lines than in EC cells. Genes expressed significantly higher in ES cell lines (black) relative to EC cell lines and in EC cell lines (gray) relative to ES cell lines were determined by direct comparison using SAM. The chromosomal location of each unique gene was retrieved from April 2003 freeze Golden Path (http://genome.ucsc.edu). The distribution of genes on the chromosomes is expressed as the ratio of the number genes per chromosome in the subset to the number of genes per chromosome in the starting microarray set illustrated in Fig. 1. Chromosome 12 in EC cells is significantly different from expected [hypergeometric test (P < 0.00001)].

Samples of the hierarchical cluster analysis of 9,976 cDNA clones that were expressed at least 3-fold different from the mean expression values across all of the samples in at least four different arrays. The colors indicates the relative expression levels of each gene, with red indicating positive expression above reference and green indicating negative expression below the reference. (A) The dendrogram represents the relationship of the expression patterns. The lengths of the arms are proportional to the similarities of the expression patterns, with shorter arms indicating closer relationships. (B) Cluster of genes surrounding POU5F1. These genes are highly expressed in ES and EC cells and seminomas but not YSCs and other differentiated tissue types. (C) Cluster of genes highly expressed in ES and EC cells but not seminomas, YSCs, or other differentiated tissues. (D) Cluster of genes including members of Wnt-β-catenin pathway expressed in ES and EC cells and YSCs. See Table 5, which is published as supporting information on the PNAS web site, or http://microarray-pubs.stanford.edu/es_cells_2/supplement.shtml for a complete list of the genes.