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Breast Cancer Res. 2003;5(6):R223-30. Epub 2003 Sep 26.

aHIF but not HIF-1alpha transcript is a poor prognostic marker in human breast cancer.

Author information

  • 1Department of Pathology and INSERM U484, Centre Jean Perrin, Clermont-Ferrand, France. anne.cayre@cjp.fr

Abstract

BACKGROUND:

Hypoxia-inducible factor-1alpha (HIF-1alpha) is part of a transcriptional factor that regulates genes involved in metabolic and vascular adaptation of tumours to oxygen restriction. A splicing variant lacking exon 14 (sHIF-1alpha) encodes a truncated protein that competes with the normal HIF-1alpha protein, decreasing its activity. A natural antisense transcript (aHIF) complementary to the 3'-untranslated region of HIF-1alpha mRNA was described recently.

METHODS:

With a semiquantitative multiplex reverse transcriptase-PCR (RT-PCR) assay, we assessed transcript concentrations of HIF-1alpha, sHIF-1alpha and aHIF in 110 patients with invasive breast carcinoma.

RESULTS:

We found a strong positive association between HIF-1alpha and sHIF-1alpha, sHIF-1alpha and aHIF, and an inverse correlation between HIF-1alpha /sHIF-1alpha and aHIF. aHIF transcript expression was associated with poor disease-free survival in univariate (P = 0.0038) and multivariate (P = 0.0016) analyses in this series of high-risk primary breast carcinomas.

CONCLUSION:

In our series of breast cancer patients, aHIF, and not HIF-1alpha transcript, is a marker of poor prognosis.

PMID:
14580258
[PubMed - indexed for MEDLINE]
PMCID:
PMC314412
Free PMC Article

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