In vivo expression of recombinant pregnancy-specific glycoprotein 1a induces alternative activation of monocytes and enhances Th2-type immune response

Eur J Immunol. 2003 Nov;33(11):3007-16. doi: 10.1002/eji.200323993.

Abstract

It has been proposed that pregnancy-specific factors could be responsible for shift the balance of cytokine profiles during maternal immune response from Th1-type reactivity into a "less-damaging" Th2-type reactivity. In the present work, we investigated the in vivo function of human pregnancy-specific glycoprotein (PSG)1a, the major variant of PSG polypeptides released into the circulation during pregnancy, on the modulation of the innate and adaptive immune response. For this, BALB/c mice were injected with a vaccinia virus-based vector harboring the human PSG1a cDNA (Vac-PSG1a) 4 days before immunization with ovalbumin (OVA) in complete Freund's adjuvant, and the early specific T cell response against OVA was evaluated 8 days post-immunization. We also studied the activation status of spleen and peritoneal monocytes/macrophages (Mo) populations from Vac-PSG1a-treated mice, and explored whether PSG1a-targeted Mo could affect the Th-type commitment by investigating their impact on the differentiation of naive T cells. Our data show that the treatment with Vac-PSG1a is able to induce a state of alternative activation on Mo. Furthermore, the generation of the immune response in the context of these alternatively activated antigen-presenting cells may shift T cell differentiation to Th2-type immunity which is more compatible with a successful pregnancy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / physiology
  • Cytokines / metabolism
  • Glycoproteins / biosynthesis
  • Glycoproteins / genetics*
  • Humans
  • Interleukin-10 / metabolism
  • Mice
  • Monocytes / immunology*
  • Pregnancy Proteins / biosynthesis
  • Pregnancy Proteins / genetics*
  • Spleen / immunology
  • Th2 Cells / immunology*
  • Th2 Cells / metabolism

Substances

  • Cytokines
  • Glycoproteins
  • Pregnancy Proteins
  • Interleukin-10