Display Settings:

Format

Send to:

Choose Destination
    Curr Opin Struct Biol. 2003 Oct;13(5):595-601.

    Peptide and protein de novo sequencing by mass spectrometry.

    Source

    Department of Physics and Astronomy, University of Manitoba, MB R3T 2N2, Winnipeg, Canada. standin@cc.umanitoba.ca

    Abstract

    Although the advent of large-scale genomic sequencing has greatly simplified the task of determining the primary structures of peptides and proteins, the genomic sequences of many organisms are still unknown. Even for those that are known, modifications such as post-translational events may prevent the identification of all or part of the protein sequence. Thus, complete characterization of the protein primary structure often requires determination of the protein sequence by mass spectrometry with minimal assistance from genomic data - de novo protein sequencing. This task has been facilitated by technical developments during the past few years: 'soft' ionization techniques, new forms of chemical modification (derivatization), new types of mass spectrometer and improved software.

    PMID:
    14568614
    [PubMed - indexed for MEDLINE]

      Supplemental Content

      Icon for Elsevier Science

      Save items

      loading

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk