Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking

Maxime Dahan; Sabine Lévi; Camilla Luccardini; Philippe Rostaing; Béatrice Riveau; Antoine Triller

doi:10.1126/science.1088525

Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking

Science. 2003 Oct 17;302(5644):442-5. doi: 10.1126/science.1088525.

Authors

Maxime Dahan¹, Sabine Lévi, Camilla Luccardini, Philippe Rostaing, Béatrice Riveau, Antoine Triller

Affiliation

¹ Laboratoire Kastler Brossel, CNRS UMR 8552, Ecole Normale Supérieure and Université Pierre et Marie Curie, 24 rue Lhomond, 75005 Paris, France. maxime.dahan@lkb.ens.fr

PMID: 14564008
DOI: 10.1126/science.1088525

Abstract

Semiconductor quantum dots (QDs) are nanometer-sized fluorescent probes suitable for advanced biological imaging. We used QDs to track individual glycine receptors (GlyRs) and analyze their lateral dynamics in the neuronal membrane of living cells for periods ranging from milliseconds to minutes. We characterized multiple diffusion domains in relation to the synaptic, perisynaptic, or extrasynaptic GlyR localization. The entry of GlyRs into the synapse by diffusion was observed and further confirmed by electron microscopy imaging of QD-tagged receptors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Membrane / metabolism
Cell Membrane / ultrastructure
Cells, Cultured
Dendrites / metabolism
Dendrites / ultrastructure
Diffusion
Fluorescence
Fluorescent Dyes*
Microscopy, Electron
Nanotechnology*
Neurites / metabolism
Neurites / ultrastructure
Neurons / metabolism*
Neurons / ultrastructure
Pyridinium Compounds
Quaternary Ammonium Compounds
Rats
Rats, Sprague-Dawley
Receptors, Glycine / metabolism*
Semiconductors
Spinal Cord / cytology
Synapses / metabolism
Synapses / ultrastructure

Substances

FM 4-64
Fluorescent Dyes
Pyridinium Compounds
Quaternary Ammonium Compounds
Receptors, Glycine