Nat Cell Biol. 2003 Sep;Suppl:S7-14.

Photobleaching and photoactivation: following protein dynamics in living cells.

Lippincott-Schwartz J, Altan-Bonnet N, Patterson GH.

Source

Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. jlippin@helix.nih.gov

Abstract

Cell biology is being transformed by the use of fluorescent proteins as fusion tags to track protein behaviour in living cells. Here, we discuss the techniques of photobleaching and photoactivation, which can reveal the location and movement of proteins. Widespread applications of these fluorescent-based methods are revealing new aspects of protein dynamics and the biological processes that they regulate.

PMID:: 14562845; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Review

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Review Photobleaching GFP reveals protein dynamics inside live cells. [Trends Cell Biol. 1999]
Review Photobleaching GFP reveals protein dynamics inside live cells.
White J, Stelzer E. Trends Cell Biol. 1999 Feb; 9(2):61-5.
Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells. [J Microsc. 2002]
Fluorescence localization after photobleaching (FLAP): a new method for studying protein dynamics in living cells.
Dunn GA, Dobbie IM, Monypenny J, Holt MR, Zicha D. J Microsc. 2002 Jan; 205(Pt 1):109-12.
Review Development and use of fluorescent protein markers in living cells. [Science. 2003]
Review Development and use of fluorescent protein markers in living cells.
Lippincott-Schwartz J, Patterson GH. Science. 2003 Apr 4; 300(5616):87-91.
Review Photoactivation and imaging of photoactivatable fluorescent proteins. [Curr Protoc Cell Biol. 2008]
Review Photoactivation and imaging of photoactivatable fluorescent proteins.
Patterson GH. Curr Protoc Cell Biol. 2008 Mar; Chapter 21:Unit 21.6.
Review Spectral imaging and its applications in live cell microscopy. [FEBS Lett. 2003]
Review Spectral imaging and its applications in live cell microscopy.
Zimmermann T, Rietdorf J, Pepperkok R. FEBS Lett. 2003 Jul 3; 546(1):87-92.

See reviews... See all...

Cited by 36 PubMed Central articles

Live-cell fluorescence correlation spectroscopy dissects the role of coregulator exchange and chromatin binding in retinoic acid receptor mobility. [J Cell Sci. 2011]
Live-cell fluorescence correlation spectroscopy dissects the role of coregulator exchange and chromatin binding in retinoic acid receptor mobility.
Brazda P, Szekeres T, Bravics B, Tóth K, Vámosi G, Nagy L. J Cell Sci. 2011 Nov 1; 124(Pt 21):3631-42. Epub 2011 Nov 1.
The nucleolus directly regulates p53 export and degradation. [J Cell Biol. 2011]
The nucleolus directly regulates p53 export and degradation.
Boyd MT, Vlatkovic N, Rubbi CP. J Cell Biol. 2011 Sep 5; 194(5):689-703.
Protein diffusion in mammalian cell cytoplasm. [PLoS One. 2011]
Protein diffusion in mammalian cell cytoplasm.
Kühn T, Ihalainen TO, Hyväluoma J, Dross N, Willman SF, Langowski J, Vihinen-Ranta M, Timonen J. PLoS One. 2011; 6(8):e22962. Epub 2011 Aug 19.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Photobleaching and photoactivation: following protein dynamics in living cells.
Photobleaching and photoactivation: following protein dynamics in living cells.
Nat Cell Biol. 2003 Sep ;Suppl:S7-14.

PubMed
Mucopolysaccharidosis type VII (beta-glucuronidase deficiency): a chronic varian...
Mucopolysaccharidosis type VII (beta-glucuronidase deficiency): a chronic variant with an oligosymptomatic severe skeletal dysplasia.
Am J Med Genet. 1992 Sep 15 ;44(2):145-52.

PubMed
Vasodilating mechanisms of levosimendan.
Vasodilating mechanisms of levosimendan.
Cardiovasc Drugs Ther. 2003 Mar ;17(2):111-3.

PubMed
Mucopolysaccharidosis VII as cause of fetal hydrops in early pregnancy.
Mucopolysaccharidosis VII as cause of fetal hydrops in early pregnancy.
Am J Med Genet. 1992 Sep 15 ;44(2):142-4.

PubMed
Prevention of prostate cancer with finasteride.
Prevention of prostate cancer with finasteride.
N Engl J Med. 2003 Oct 16 ;349(16):1569-72; author reply 1569-72.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: