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J Assist Reprod Genet. 2003 Sep;20(9):358-64.

The effect of preincubation period of oocytes on nuclear maturity, fertilization rate, embryo quality, and pregnancy outcome in IVF and ICSI.

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  • 1Department of Obstetrics and Gynecology, Taichung Veterans General Hospital, 160, Sec. 3, Chung-Kang Road, Taichung 407, Taiwan, Republic of China.

Abstract

PURPOSE:

To clarify the effect of preincubation of oocytes on the results of IVF and ICSI.

METHODS:

A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm; fertilization rates, embryo quality, and pregnancy outcomes were analyzed in both IVF and ICSI arms according to the preincubation period duration of oocytes.

RESULTS:

The MII percentage of the ICSI arm was significantly lower (P < 0.05) in the group with preincubation period of < 2.5 h. The fertilization rates in groups with preincubation for 2.5-5.5 h were significantly higher (P < 0.001) for IVF. Embryo quality and pregnancy outcomes were not significantly different between the IVF or ICSI arm.

CONCLUSIONS:

The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.

PMID:
14531646
PMCID:
PMC3455840
[PubMed - indexed for MEDLINE]
Free PMC Article
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