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Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12319-24. Epub 2003 Oct 6.

Systemic and cell type-specific gene expression patterns in scleroderma skin.

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  • 1Department of Dermatology, University of California-San Francisco, San Francisco, CA 94143, USA.

Abstract

We used DNA microarrays representing >12,000 human genes to characterize gene expression patterns in skin biopsies from individuals with a diagnosis of systemic sclerosis with diffuse scleroderma. We found consistent differences in the patterns of gene expression between skin biopsies from individuals with scleroderma and those from normal, unaffected individuals. The biopsies from affected individuals showed nearly indistinguishable patterns of gene expression in clinically affected and clinically unaffected tissue, even though these were clearly distinguishable from the patterns found in similar tissue from unaffected individuals. Genes characteristically expressed in endothelial cells, B lymphocytes, and fibroblasts showed differential expression between scleroderma and normal biopsies. Analysis of lymphocyte populations in scleroderma skin biopsies by immunohistochemistry suggest the B lymphocyte signature observed on our arrays is from CD20+ B cells. These results provide evidence that scleroderma has systemic manifestations that affect multiple cell types and suggests genes that could be used as potential markers for the disease.

PMID:
14530402
[PubMed - indexed for MEDLINE]
PMCID:
PMC218756
Free PMC Article

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