Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Genes Dev. 2003 Oct 15;17(20):2526-38. Epub 2003 Oct 1.

ELAV inhibits 3'-end processing to promote neural splicing of ewg pre-mRNA.

Author information

  • 1Department of Biology and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454, USA. msoller@brandeis.edu

Abstract

The embryonic lethal abnormal visual system (ELAV) is a gene-specific regulator of alternative pre-mRNA processing in neurons of Drosophila. Here we define a functional in vivo binding site for ELAV in neurons through the development of a reporter gene system in transgenic animals in combination with in vitro binding assays. ELAV binds to erect wing (ewg) RNA 3' of a polyadenylation site in the terminal intron 6. At this polyadenylation site, ELAV inhibits 3'-end processing in vitro in a dose-dependent and sequence-specific manner, and ELAV binding is necessary in vivo to promote splicing of ewg intron 6. Further, the AAUAAA poly(A) complex recognition sequence, together with ELAV, is required to regulate neural 3' splice site choice in vivo. In addition, the use of segmentally labeled RNA substrates in UV cross-linking assays suggest that ELAV does not inhibit or redirect binding of cleavage factor dCstF64 at the regulated polyadenylation site on ewg RNA. These data indicate that binding of 3'-end processing factors, together with ELAV, can regulate alternative splicing.

PMID:
14522950
[PubMed - indexed for MEDLINE]
PMCID:
PMC218147
Free PMC Article

Images from this publication.See all images (7)Free text

Figure 1.
Figure 2.
Figure 7.
Figure 3.
Figure 6.
Figure 4.
Figure 5.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk