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1: Biosci Biotechnol Biochem. 2003 Sep;67(9):2034-7. Links

A simple and eficient method for high fidelity PCR cloning using antibody-neutralizing technology.

Kitabayashi M, Nishiya Y, Esaka M.

Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.

We introduce the TA cloning antibody method for the high-fidelity PCR product amplified by family B DNA polymerase without purification. This method uses antibodies and Thermus aquaticus (Taq) DNA polymerase. The antibodies can inhibit only the activity of family B DNA polymerase, and Taq can co-work for A-tailing. This method has nearly cloning efficiency to that of the PCR product of Taq.

PMID: 14519999 [PubMed - indexed for MEDLINE]