Subsite structure of maltogenic amylase from Thermomonospora viridis TF-35

Biosci Biotechnol Biochem. 2003 Sep;67(9):1993-5. doi: 10.1271/bbb.67.1993.

Abstract

We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A(-3), A(-2), (A(-1)+A(+1)), A(+2) and A(+3) were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol(-1), respectively.

MeSH terms

  • Actinomycetales / enzymology*
  • Binding Sites
  • Catalytic Domain
  • Glycoside Hydrolases / chemistry*
  • Glycoside Hydrolases / metabolism
  • Kinetics
  • Oligosaccharides / chemistry
  • Oligosaccharides / metabolism
  • Protein Binding
  • Thermodynamics

Substances

  • Oligosaccharides
  • maltooligosaccharides
  • Glycoside Hydrolases
  • glucan 1,4-alpha-maltohydrolase