Abstract
We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A(-3), A(-2), (A(-1)+A(+1)), A(+2) and A(+3) were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol(-1), respectively.
MeSH terms
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Actinomycetales / enzymology*
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Binding Sites
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Catalytic Domain
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Glycoside Hydrolases / chemistry*
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Glycoside Hydrolases / metabolism
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Kinetics
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Oligosaccharides / chemistry
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Oligosaccharides / metabolism
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Protein Binding
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Thermodynamics
Substances
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Oligosaccharides
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maltooligosaccharides
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Glycoside Hydrolases
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glucan 1,4-alpha-maltohydrolase