Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

Opt Lett. 2003 Oct 1;28(19):1742-4. doi: 10.1364/ol.28.001742.

Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2.

MeSH terms

  • Animals
  • Fluorescent Dyes
  • Fura-2
  • Lasers*
  • Microscopy*
  • Microscopy, Fluorescence
  • Myocytes, Cardiac / ultrastructure*
  • Photons*
  • Rabbits
  • Time Factors

Substances

  • Fluorescent Dyes
  • Fura-2