Temporal profile of tissue plasminogen activator (tPA) and inhibitor expression after transient focal cerebral ischemia

Neuroreport. 2003 Sep 15;14(13):1689-92. doi: 10.1097/00001756-200309150-00006.

Abstract

Altered tPA expression may influence the fate of neurons after cerebral ischemia. We determined the changes in tPA and plasminogen activator inhibitor (PAI-1) expression in adult rat brain after transient middle cerebral artery (MCA) occlusion. Immunohistology revealed tPA staining in ipsilateral but not contralateral cortex and striatum 6 h after occlusion. This pattern was maintained at 24 h. Staining data was supported by Western blot data which showed no tPA protein in contralateral cortex at 3 h but abundant protein in ipsilateral cortex which increased further at 6 h and 24 h. In contrast there was prominent PAI-1 immunostaining and protein expression in control tissue after MCA occlusion but it diminished progressively at 3, 6 and 24 h in the lesioned cortex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Brain / metabolism*
  • Brain Ischemia / metabolism*
  • Cerebral Cortex / metabolism
  • Corpus Striatum / metabolism
  • Functional Laterality
  • Immunohistochemistry
  • Ligation
  • Middle Cerebral Artery / surgery
  • Plasminogen Activator Inhibitor 1 / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors
  • Tissue Plasminogen Activator / metabolism*

Substances

  • Plasminogen Activator Inhibitor 1
  • Tissue Plasminogen Activator