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Methods Mol Biol. 2003;236:3-20.

An improved method for plant BAC library construction.

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  • 1Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ, USA.

Abstract

Large genomic DNA insert-containing libraries are required as critical tools for physical mapping, positional cloning, and genome sequencing of complex genomes. The bacterial artificial chromosome (BAC) cloning system has become a dominant system over others to clone large genomic DNA inserts. As the costs of positional cloning, physical mapping, and genome sequencing continuously decrease, there is an increasing demand for high-quality deep-coverage large insert BAC libraries. In our laboratory, we have constructed many high-quality deep-coverage large insert BAC libraries including arabidopsis, manocot and dicot crop plants, and plant pathogens. Here, we present the protocol used in our laboratory to construct BAC libraries.

PMID:
14501055
[PubMed - indexed for MEDLINE]
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