[Confocal laser scanning microscopy for image cytometry]

Nihon Rinsho. 1992 Oct;50(10):2542-6.
[Article in Japanese]

Abstract

By using a focused laser beam as the light source of the microscope, to minimized flare, together with a pinhole in front of a photo detector to eliminate out-of-focus data, the confocal laser scanning microscope (CLSM) provides a depth-discriminated fluorescence image with high spatial resolution. The CLSM, therefore, has been widely used as a tool for accurate morphometric and densitometric analyses. We discussed here the biological applications of CLSM for demonstrating the three-dimensional features of the embryonic heart tube and chromosome and for imaging fast dynamic changes of [Ca2+]i in the living heart muscle cell pairs.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Animals
  • Calcium / metabolism
  • Chromosomes
  • Cytophotometry / instrumentation*
  • Lasers*
  • Microscopy, Electron, Scanning*
  • Myocardium / metabolism

Substances

  • Calcium