Science. 1992 Nov 6;258(5084):972-5.

Chemical contrast in X-ray microscopy and spatially resolved XANES spectroscopy of organic specimens.

Ade H, Zhang X, Cameron S, Costello C, Kirz J, Williams S.

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Department of Physics, State University of New York, Stony Brook, NY 11794.

Abstract

The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.

PMID:: 1439809; [PubMed - indexed for MEDLINE]

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