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Nature. 1992 Nov 12;360(6400):127-34.

Structure of a C-type mannose-binding protein complexed with an oligosaccharide.

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  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032.

Abstract

C-type (Ca(2+)-dependent) animal lectins such as mannose-binding proteins mediate many cell-surface carbohydrate-recognition events. The crystal structure at 1.7 A resolution of the carbohydrate-recognition domain of rat mannose-binding protein complexed with an oligomannose asparaginyl-oligosaccharide reveals that Ca2+ forms coordination bonds with the carbohydrate ligand. Carbohydrate specificity is determined by a network of coordination and hydrogen bonds that stabilizes the ternary complex of protein, Ca2+ and sugar. Two branches of the oligosaccharide crosslink neighbouring carbohydrate-recognition domains in the crystal, enabling multivalent binding to a single oligosaccharide chain to be visualized directly.

[PubMed - indexed for MEDLINE]
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