A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay for the detection of rhabdovirus of penaeid shrimps from infected animals

E C Nadala Jr; Y Lu; P C Loh; J A Brock

doi:10.1016/0166-0934(92)90141-y

A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay for the detection of rhabdovirus of penaeid shrimps from infected animals

J Virol Methods. 1992 Sep;39(1-2):227-9. doi: 10.1016/0166-0934(92)90141-y.

Authors

E C Nadala Jr¹, Y Lu, P C Loh, J A Brock

Affiliation

¹ Department of Microbiology, University of Hawaii, Honolulu.

PMID: 1430062
DOI: 10.1016/0166-0934(92)90141-y

Abstract

A streptavidin-biotin-enhanced nitrocellulose enzyme immunoassay was developed for the detection of the rhabdovirus of penaeid shrimps (RPS) in the tissues of infected animals. Initial tests indicate that the assay was capable of detecting as few as ten plaque-forming units of virus.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Proteins
Biotin
Collodion
Decapoda / microbiology*
Evaluation Studies as Topic
Immunoenzyme Techniques*
Rhabdoviridae / isolation & purification*
Streptavidin
Viral Plaque Assay / methods
Virology / methods

Substances

Bacterial Proteins
Biotin
Collodion
Streptavidin