Sodium dodecyl sulfate-capillary gel electrophoresis of proteins using non-cross-linked polyacrylamide

J Chromatogr. 1992 Sep 11;608(1-2):349-56. doi: 10.1016/0021-9673(92)87142-u.

Abstract

Proteins with relative molecular masses of 14,000 to 205,000 were separated by sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) using non-cross-linked linear polyacrylamide gels on both coated and uncoated fused-silica capillaries. It was determined that viscosity of the acrylamide solution was a major factor affecting column stability with linear acrylamide gels. When the viscosity of the acrylamide solution reaches 100 cP, electro-osmotically driven displacement of the gels is insignificant. Uncoated capillaries provided better resolution, stability, and reproducibility than surface coated capillaries when the concentration of linear polyacrylamide was greater than 4%. At lower gel concentrations, non-cross-linked polyacrylamide is easily displaced from the columns. A calibration plot of log molecular mass vs. mobility with non-linear polyacrylamide was linear, which indicated that resolution was equivalent to that obtained with cross-linked acrylamide. Separations with model proteins indicated that baseline resolution between protein species that vary 10% in molecular mass can be achieved.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acrylic Resins / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunoglobulin G / analysis
  • Molecular Weight
  • Myoglobin / analysis
  • Proteins / analysis*
  • Viscosity

Substances

  • Acrylic Resins
  • Immunoglobulin G
  • Myoglobin
  • Proteins
  • polyacrylamide