AMINO GROUP FORMATION AND GLUTAMATE SYNTHESIS IN STREPTOCOCCUS BOVIS

J Bacteriol. 1964 Oct;88(4):1038-44. doi: 10.1128/jb.88.4.1038-1044.1964.

Abstract

Burchall, J. J. (University of Illinois, Urbana), R. A. Niederman, and M. J. Wolin. Amino group formation and glutamate synthesis in Streptococcus bovis. J. Bacteriol. 88:1038-1044. 1964.-Extracts of Streptococcus bovis grown on NH(4) (+) as a nitrogen source contain a nicotinamide adenine dinucleotide phosphate (NADP)-linked glutamic dehydrogenase and are devoid of alanine dehydrogenase, other amino acid dehydrohygenases, and aspartase. A potential source of reduced nicotinamide adenine dinucleotide phosphate for glutamate synthesis is a NADP and nicotinamide adenine dinucleotide (NAD)-linked glyceraldehyde-3-phosphate dehydrogenase present in the extracts. Experiments with C(14)-labeled glucose and NaHCO(3) indicate that the glutamate carbon skeleton is synthesized by a tricarboxylic acid pathway. The synthesis of the carbon skeleton of glutamate is repressed when glutamate or casein hydrolysate supplement the NH(4) (+)-containing growth medium. Repression of glutamic dehydrogenase and a NAD-linked isocitric dehydrogenase occurs only when complex nitrogen sources, but not when free amino acids, are added to the growth medium.

MeSH terms

  • Amino Acids*
  • Asparagine*
  • Aspartic Acid*
  • Carbon*
  • Caseins*
  • Culture Media*
  • Glutamate Dehydrogenase*
  • Glutamates*
  • Glutamic Acid*
  • Glyceraldehyde-3-Phosphate Dehydrogenases*
  • Isocitrate Dehydrogenase*
  • Metabolism*
  • NAD*
  • NADP*
  • Nitrogen*
  • Nucleotides*
  • Research*
  • Streptococcus bovis*
  • Streptococcus*

Substances

  • Amino Acids
  • Caseins
  • Culture Media
  • Glutamates
  • Nucleotides
  • NAD
  • Aspartic Acid
  • Glutamic Acid
  • NADP
  • casein hydrolysate
  • Asparagine
  • Carbon
  • Isocitrate Dehydrogenase
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Glutamate Dehydrogenase
  • Nitrogen