J Infect Dis. 1992 Nov;166(5):1146-8.

Diagnosis of vertical human immunodeficiency virus type 1 infection by whole blood culture.

Alimenti A, O'Neill M, Sullivan JL, Luzuriaga K.

Source

Department of Pediatrics, University of Massachusetts Medical Center, Worcester 01605.

Abstract

The qualitative and quantitative performance of a human immunodeficiency virus type 1 (HIV-1) whole blood culture method was assessed for use in the diagnosis of vertical HIV-1 infection. This method requires < 1 mL of whole blood and allows for the quantification of blood virus load. HIV-1 was isolated from 36 (95%) of 38 whole blood specimens from 27 HIV-1-infected children compared with 0 of 16 whole blood specimens from 16 uninfected children. HIV-1 titers were significantly higher in severely symptomatic children (2450 TCID/mL) than in less symptomatic children (179 TCID/mL). This simple quantitative culture assay may be useful for the early diagnosis of vertical HIV-1 infection, the assessment of blood virus load, and the evaluation of antiretroviral therapies.

PMID:: 1402027; [PubMed - indexed for MEDLINE]

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Zidovudine

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AI-32391/AI/NIAID NIH HHS/United States

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