Immunobiology. 1992 Jun;185(1):78-89.

Measurement of lymphocyte proliferation: critical analysis of radioactive and photometric methods.

Wemme H, Pfeifer S, Heck R, Müller-Quernheim J.

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Third Department of Internal Medicine, Johannes Gutenberg University, Mainz, Germany.

Abstract

Different methods of lymphocyte proliferation are compared to identify a non-radioactive alternative to 3H-thymidine-test. The enzymatic assays evaluating the turnover of mitochondrial dehydrogenases (MTT-test) and lysosomal hexosaminidase (NAG-test) proved not sensitive enough to substitute for 3H-thymidine incorporation. The incorporation of the nucleotide analog 5-bromodeoxyuridine (BrdU) can be exploited using an ELISA-system (enzyme linked immunosorbent assay) employing a monoclonal anti-BrdU antibody to measure cell proliferation. An optimized test protocol of the BrdU-ELISA which fulfills the requirements for a sensitive and practicable non-radioactive alternative to 3H-thymidine-test is presented.

PMID:: 1398743; [PubMed - indexed for MEDLINE]

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Measurement of lymphocyte proliferation: critical analysis of radioactive and photometric methods.

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