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FEBS Lett. 1992 Sep 21;310(1):63-5.

Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues.

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  • 1Department of Biochemistry, University of Oxford, UK.

Abstract

The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked.

PMID:
1388125
[PubMed - indexed for MEDLINE]
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